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ELK Biotechnology

ELK5315

Rat CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit

Rat CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Rat CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit

Overview

Product name: Rat LL-37(Antibacterial Protein LL-37) ELISA Kit
Reactivity: Rat
Alternative Names: CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic; CAMP; Cathelicidin Antimicrobial Peptide
Assay Type: Competitive Inhibition
Sensitivity: 9.16 ng/mL
Standard: 2000 ng/mL
Range: 31.25-2000 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Infection immunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat LL-37 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
2000.00 0.212 —
1000.00 0.385 —
500.00 0.575 —
250.00 0.895 —
125.00 1.168 —
62.50 1.585 —
31.25 1.624 —
0.00 2.256 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant LL-37 and the recovery rates were calculated by comparing the measured value to the expected amount of LL-37 in samples.
Matrix Recovery range Average
serum(n=5) 90-103% 96%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 85-97% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LL-37 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 80-92% 86-102% 94-106% 85-97%
EDTA plasma(n=5) 96-101% 85-92% 79-96% 89-101%
Heparin plasma(n=5) 86-102% 86-101% 87-96% 92-105%
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