1 1

ELK Biotechnology

ELK5157

Human ACTL6A(Actin Like Protein 6A) ELISA Kit

Human ACTL6A(Actin Like Protein 6A) ELISA Kit

$458.00 USD
$458.00 USD
Sale Sold out
Shipping calculated at checkout.
Size
Sensitivity
Detection range

Human ACTL6A(Actin Like Protein 6A) ELISA Kit

Overview

Product name: Human ACTL6A(Actin Like Protein 6A) ELISA Kit
Reactivity: Human
Alternative Names: Actl6; BAF53A; Arp4; Baf53a; INO80K; BAF Complex 53 kDa Subunit; 53 kDa BRG1-associated factor A; Actin-Related Protein 4; INO80 Complex Subunit K
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACTL6A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACTL6A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACTL6A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACTL6A in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 1.994 1.843
5.00 1.577 1.426
2.50 1.184 1.033
1.25 0.756 0.605
0.63 0.498 0.347
0.32 0.352 0.201
0.16 0.235 0.084
0.00 0.151 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ACTL6A and the recovery rates were calculated by comparing the measured value to the expected amount of ACTL6A in samples.
Matrix Recovery range Average
serum(n=5) 89-101% 95%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 80-94% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTL6A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-93% 78-91% 87-98% 95-107%
EDTA plasma(n=5) 85-94% 93-101% 87-98% 89-102%
Heparin plasma(n=5) 78-91% 86-92% 89-96% 91-97%
View full details