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ELK Biotechnology

ELK5115

Human CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit

Human CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit

Overview

Product name: Human LL-37(Antibacterial Protein LL-37) ELISA Kit
Reactivity: Human
Alternative Names: CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic; CAMP; Cathelicidin Antimicrobial Peptide
Assay Type: Competitive Inhibition
Sensitivity: 49.2 ng/mL
Standard: 10000 ng/mL
Range: 156.25-10000 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Infection immunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human LL-37 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10000.00 0.237 —
5000.00 0.427 —
2500.00 0.786 —
1250.00 0.967 —
625.00 1.275 —
312.50 1.565 —
156.25 1.835 —
0.00 2.265 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant LL-37 and the recovery rates were calculated by comparing the measured value to the expected amount of LL-37 in samples.
Matrix Recovery range Average
serum(n=5) 92-106% 99%
EDTA plasma(n=5) 87-95% 91%
Heparin plasma(n=5) 78-92% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LL-37 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 79-93% 78-90% 91-99%
EDTA plasma(n=5) 80-92% 86-102% 87-103% 85-101%
Heparin plasma(n=5) 88-99% 87-103% 89-97% 85-94%
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