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ELK Biotechnology

ELK5006

Human BRD1(Bromodomain Containing Protein 1) ELISA Kit

Human BRD1(Bromodomain Containing Protein 1) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human BRD1(Bromodomain Containing Protein 1) ELISA Kit

Overview

Product name: Human BRD1(Bromodomain Containing Protein 1) ELISA Kit
Reactivity: Human
Alternative Names: BRL; BRPF2; BR140-like protein; Bromodomain and PHD finger-containing protein 2
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
Standard: 10 ng/mL
Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BRD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BRD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.121 2.029
5.00 1.721 1.629
2.50 1.073 0.981
1.25 0.733 0.641
0.63 0.509 0.417
0.32 0.313 0.221
0.16 0.175 0.083
0.00 0.092 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant BRD1 and the recovery rates were calculated by comparing the measured value to the expected amount of BRD1 in samples.
Matrix Recovery range Average
serum(n=5) 92-106% 99%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 88-103% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BRD1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 91-99% 83-89% 88-94% 87-93%
EDTA plasma(n=5) 85-104% 83-96% 96-102% 88-95%
Heparin plasma(n=5) 87-92% 85-94% 89-98% 85-94%
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