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ELK Biotechnology

ELK4964

Human HIF1aN(Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor) ELISA Kit

Human HIF1aN(Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human HIF1aN(Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor) ELISA Kit

Overview

Product name: Human HIF1aN(Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor) ELISA Kit
Reactivity: Human
Alternative Names: FIH1; Peptide-Aspartate Beta-Dioxygenase; Hypoxia-inducible factor asparagine hydroxylase
Assay Type: Sandwich
Sensitivity: 14.4 pg/mL
Standard: 2000 pg/mL
Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HIF1aN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HIF1aN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HIF1aN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HIF1aN in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
2000.00 2.013 1.925
1000.00 1.602 1.514
500.00 1.235 1.147
250.00 0.963 0.875
125.00 0.508 0.420
62.50 0.357 0.269
31.25 0.258 0.170
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant HIF1aN and the recovery rates were calculated by comparing the measured value to the expected amount of HIF1aN in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 92-105% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF1aN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-101% 86-97% 88-95%
EDTA plasma(n=5) 92-101% 87-98% 85-92% 79-96%
Heparin plasma(n=5) 85-103% 82-96% 87-105% 90-99%
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