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ELK Biotechnology

ELK4908

Chicken sIgA(Secretory Immunoglobulin A) ELISA Kit

Chicken sIgA(Secretory Immunoglobulin A) ELISA Kit

$588.00 USD
$588.00 USD
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Size
Sensitivity
Detection range

Chicken sIgA(Secretory Immunoglobulin A) ELISA Kit

Overview

Product name: Chicken sIgA(Secretory Immunoglobulin A) ELISA Kit
Reactivity: Chicken
Alternative Names: S-IgA
Assay Type: Competitive Inhibition
Sensitivity: 248.5 pg/mL
Standard: 50000 pg/mL
Range: 781.25-50000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Infection immunity;Immune molecule;Reproductive science;Genetic science;Dermatology;Immunodeficiency;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken sIgA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken sIgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken sIgA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50000.00 0.212 —
25000.00 0.386 —
12500.00 0.583 —
6250.00 0.833 —
3125.00 1.197 —
1562.50 1.511 —
781.25 1.715 —
0.00 2.213 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sIgA and the recovery rates were calculated by comparing the measured value to the expected amount of sIgA in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 81-95% 88%
Heparin plasma(n=5) 94-105% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sIgA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-99% 93-107% 89-97% 85-94%
EDTA plasma(n=5) 87-96% 85-97% 85-94% 87-96%
Heparin plasma(n=5) 90-99% 89-102% 87-98% 85-92%
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