1 1

ELK Biotechnology

ELK4897

Rat Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit

Rat Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit

$458.00 USD
$458.00 USD
Sale Sold out
Shipping calculated at checkout.
Size
Sensitivity
Detection range

Rat Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit

Overview

Product name: Rat Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit
Reactivity: Rat
Alternative Names: Amyloid beta 42(ABeta 42); amyloid beta peptide 1-42; Aβ1-42,amyloid βpeptide 1-42
Assay Type: Sandwich
Sensitivity: 4.54 pg/mL
Standard: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 1.983 1.890
500.00 1.636 1.543
250.00 1.243 1.150
125.00 0.769 0.676
62.50 0.527 0.434
31.25 0.381 0.288
15.63 0.187 0.094
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Ab1-42 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-42 in samples.
Matrix Recovery range Average
serum(n=5) 84-97% 90%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 77-95% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-42 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 91-99% 83-95% 89-97%
EDTA plasma(n=5) 85-94% 78-96% 79-93% 82-90%
Heparin plasma(n=5) 95-102% 89-96% 87-98% 85-93%
View full details