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ELK Biotechnology

ELK4760

Rat IgG1(Immunoglobulin G1) ELISA Kit

Rat IgG1(Immunoglobulin G1) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Rat IgG1(Immunoglobulin G1) ELISA Kit

Overview

Product name: Rat IgG1(Immunoglobulin G1) ELISA Kit
Reactivity: Rat
Alternative Names: IGHG1; Ig Gamma-1 Chain C Region; Immunoglobulin Heavy Constant Gamma 1; G1m Marker; Immunoglobulin Gm1
Assay Type: Competitive Inhibition
Sensitivity: 18.7 ng/mL
Standard: 4000 ng/mL
Range: 62.5-4000 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Immune molecule;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat IgG1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IgG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IgG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
4000.00 0.227 —
2000.00 0.386 —
1000.00 0.596 —
500.00 0.823 —
250.00 1.169 —
125.00 1.537 —
62.50 1.697 —
0.00 2.112 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IgG1 and the recovery rates were calculated by comparing the measured value to the expected amount of IgG1 in samples.
Matrix Recovery range Average
serum(n=5) 83-97% 90%
EDTA plasma(n=5) 87-95% 91%
Heparin plasma(n=5) 81-95% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-96% 87-101% 89-101% 85-97%
EDTA plasma(n=5) 92-105% 82-92% 86-94% 81-95%
Heparin plasma(n=5) 91-98% 89-102% 87-98% 80-92%
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