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ELK Biotechnology

ELK4683

Human PNOC(Pronociceptin) ELISA Kit

Human PNOC(Pronociceptin) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human PNOC(Pronociceptin) ELISA Kit

Overview

Product name: Human OFQ/N(OrphaninFQ/Nociceptin) ELISA Kit
Reactivity: Human
Alternative Names: PPNOC; Prepronociceptin; Pocistatin; Pro-nociceptin; Preproorphanin FQ; Neuropeptide 1; Nociceptin; Orphanin FQ; PNOC; Pronociceptin
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OFQ/N. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OFQ/N. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OFQ/N, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OFQ/N in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.058 1.969
5.00 1.616 1.527
2.50 1.121 1.032
1.25 0.883 0.794
0.63 0.593 0.504
0.32 0.326 0.237
0.16 0.288 0.199
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant OFQ/N and the recovery rates were calculated by comparing the measured value to the expected amount of OFQ/N in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 97-105% 101%
Heparin plasma(n=5) 83-99% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OFQ/N and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 78-92% 87-98% 95-102%
EDTA plasma(n=5) 79-97% 96-104% 85-93% 89-97%
Heparin plasma(n=5) 85-94% 87-103% 97-105% 79-90%
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