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    ELK Biotechnology

    ELK3707

    Human CACNa1D(Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit) ELISA Kit

    Human CACNa1D(Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit) ELISA Kit

    $458.00 USD
    $458.00 USD
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    Detection range

    Human CACNa1D(Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit) ELISA Kit

    Overview

    Product name: Human CACNa1D(Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit) ELISA Kit
    Reactivity: Human
    Alternative Names: CACH3; CACN4; CACNL1A2; CCHL1A2; Cav1.3; Voltage-gated calcium channel subunit alpha Cav1.3; Calcium channel, L type, alpha-1 polypeptide, isoform 2
    Assay Type: Sandwich
    Sensitivity: 0.121 ng/mL
    Standard: 20 ng/mL
    Detection Range: 0.32-20 ng/mL
    Sample Type: Tissue homogenates and other biological fluids.
    Assay Length: 3.5h
    Research Area: Signal transduction;
    Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CACNa1D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CACNa1D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CACNa1D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CACNa1D in the samples is then determined by comparing the OD of the samples to the standard curve.

    Standard curve

    Concentration (ng/mL) OD Corrected OD
    20.00 1.950 1.865
    10.00 1.720 1.635
    5.00 1.139 1.054
    2.50 0.792 0.707
    1.25 0.518 0.433
    0.63 0.293 0.208
    0.32 0.187 0.102
    0.00 0.085 0.000

    Precision

    Intra-assay Precision (Precision within an assay)：CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Inter-assay Precision (Precision between assays)：CV%<10%

    Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

    Recovery

    Matrices listed below were spiked with certain level of recombinant CACNa1D and the recovery rates were calculated by comparing the measured value to the expected amount of CACNa1D in samples.
    Matrix Recovery range Average
    serum(n=5) 87-95% 91%
    EDTA plasma(n=5) 82-97% 89%
    Heparin plasma(n=5) 80-95% 87%

    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CACNa1D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
    Matrix 1:2 1:4 1:8 1:16
    serum(n=5) 98-106% 86-102% 88-96% 89-97%
    EDTA plasma(n=5) 85-94% 87-103% 82-94% 78-90%
    Heparin plasma(n=5) 90-99% 97-106% 95-102% 87-98%
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