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ELK Biotechnology

ELK3183

Human CD83(Cluster Of Differentiation 83) ELISA Kit

Human CD83(Cluster Of Differentiation 83) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human CD83(Cluster Of Differentiation 83) ELISA Kit

Overview

Product name: Human CD83(Cluster Of Differentiation 83) ELISA Kit
Reactivity: Human
Alternative Names: BL11; HB15; Activated B Lymphocytes,Immunoglobulin; B-cell activation protein; Cell surface protein HB15
Assay Type: Sandwich
Sensitivity: 1.42 pg/mL
Standard: 200 pg/mL
Detection Range: 3.13-200 pg/mL
Sample Type: Serum, plasma, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Infection immunity;Immune molecule;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD83. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD83. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD83, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD83 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.218 2.132
100.00 1.573 1.487
50.00 0.987 0.901
25.00 0.823 0.737
12.50 0.601 0.515
6.25 0.389 0.303
3.13 0.252 0.166
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CD83 and the recovery rates were calculated by comparing the measured value to the expected amount of CD83 in samples.
Matrix Recovery range Average
serum(n=5) 78-90% 84%
EDTA plasma(n=5) 82-97% 89%
Heparin plasma(n=5) 91-105% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD83 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 79-96% 98-105% 82-93%
EDTA plasma(n=5) 85-92% 96-104% 90-98% 80-92%
Heparin plasma(n=5) 93-101% 86-92% 81-93% 79-95%
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