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ELK Biotechnology

ELK3101

General fT3(Free Triiodothyronine) ELISA Kit

General fT3(Free Triiodothyronine) ELISA Kit

$376.00 USD
$376.00 USD
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Detection range

General fT3(Free Triiodothyronine) ELISA Kit

Overview

Product name: fT3(Free Triiodothyronine) ELISA Kit
Reactivity: General
Alternative Names: FT3
Assay Type: Competitive Inhibition
Sensitivity: 258.3 fg/mL
Standard: 60000 fg/mL
Range: 937.5-60000 fg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Signal transduction;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with fT3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to fT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
60000.00 0.197 —
30000.00 0.385 —
15000.00 0.567 —
7500.00 0.878 —
3750.00 1.139 —
1875.00 1.537 —
937.50 1.624 —
0.00 2.256 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant fT3 and the recovery rates were calculated by comparing the measured value to the expected amount of fT3 in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 94-107% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of fT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-96% 82-95% 82-93%
EDTA plasma(n=5) 85-98% 88-104% 86-102% 79-93%
Heparin plasma(n=5) 85-92% 80-96% 87-98% 95-102%
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