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ELK Biotechnology

ELK2890

Rabbit HCII(Heparin Cofactor II) ELISA Kit

Rabbit HCII(Heparin Cofactor II) ELISA Kit

$588.00 USD
$588.00 USD
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Size
Sensitivity
Detection range

Rabbit HCII(Heparin Cofactor II) ELISA Kit

Overview

Product name: Rabbit HCII(Heparin Cofactor II) ELISA Kit
Reactivity: Rabbit
Alternative Names: SERPIND1; HC2; HCF2; HC II; HLS2; LS2; Serpin D1; Serpin Peptidase Inhibitor Clade D Member 1; Protease inhibitor leuserpin-2
Assay Type: Sandwich
Sensitivity: 1.03 U/L
Standard: 200 U/L
Range: 3.13-200 U/L
Sample Type: Plasma.
Assay Length: 3.5h
Research Area: Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit HCII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit HCII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit HCII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit HCII in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.108 2.000
100.00 1.497 1.389
50.00 1.069 0.961
25.00 0.782 0.674
12.50 0.435 0.327
6.25 0.303 0.195
3.13 0.241 0.133
0.00 0.108 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant HCII and the recovery rates were calculated by comparing the measured value to the expected amount of HCII in samples.
Matrix Recovery range Average
serum(n=5) 83-97% 90%
EDTA plasma(n=5) 78-97% 87%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HCII and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 85-96% 92-102% 81-93%
EDTA plasma(n=5) 93-105% 79-86% 87-103% 85-97%
Heparin plasma(n=5) 87-90% 87-98% 85-96% 85-95%
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