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ELK Biotechnology

ELK2681

Rat PTGS2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Rat PTGS2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Rat PTGS2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Overview

Product name: Rat PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit
Reactivity: Rat
Alternative Names: PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2; Prostaglandin Endoperoxide Synthase 2
Assay Type: Sandwich
Sensitivity: 0.065 ng/mL
Standard: 10 ng/mL
Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity;Reproductive science;Pulmonology;Gastroenterology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.214 2.121
5.00 1.784 1.691
2.50 1.128 1.035
1.25 0.901 0.808
0.63 0.567 0.474
0.32 0.335 0.242
0.16 0.197 0.104
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PTGS2/COX-2 and the recovery rates were calculated by comparing the measured value to the expected amount of PTGS2/COX-2 in samples.
Matrix Recovery range Average
serum(n=5) 82-97% 89%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 87-95% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PTGS2/COX-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 85-97% 85-94% 87-96%
EDTA plasma(n=5) 85-97% 87-96% 85-96% 85-95%
Heparin plasma(n=5) 86-96% 93-104% 89-97% 85-94%
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