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ELK Biotechnology

ELK2444

Human EDN1(Endothelin 1) ELISA Kit

Human EDN1(Endothelin 1) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human EDN1(Endothelin 1) ELISA Kit

Overview

Product name: Human ET-1(Endothelin1) ELISA Kit
Reactivity: Human
Alternative Names: ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
Assay Type: Sandwich
Sensitivity: 2.71 pg/mL
Standard: 500 pg/mL
Range: 7.82-500 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
500.00 2.057 1.951
250.00 1.498 1.392
125.00 0.983 0.877
62.50 0.755 0.649
31.25 0.507 0.401
15.63 0.329 0.223
7.82 0.208 0.102
0.00 0.106 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ET-1 and the recovery rates were calculated by comparing the measured value to the expected amount of ET-1 in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 90-105% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ET-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-91% 85-93% 89-97% 85-93%
EDTA plasma(n=5) 89-97% 85-94% 87-96% 85-97%
Heparin plasma(n=5) 85-94% 87-96% 92-101% 82-98%
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