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ELK Biotechnology

ELK1503

Rat NOS2(Nitric Oxide Synthase 2, Inducible) ELISA Kit

Rat NOS2(Nitric Oxide Synthase 2, Inducible) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Rat NOS2(Nitric Oxide Synthase 2, Inducible) ELISA Kit

OverviewPublications

Product name: Rat NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit
Reactivity: Rat
Alternative Names: NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible
Assay Type: Sandwich
Sensitivity: 0.62 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 1.979 1.883
50.00 1.562 1.466
25.00 1.029 0.933
12.50 0.885 0.789
6.25 0.561 0.465
3.13 0.357 0.261
1.57 0.268 0.172
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NOS2/iNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS2/iNOS in samples.
Matrix Recovery range Average
serum(n=5) 81-97% 89%
EDTA plasma(n=5) 84-97% 90%
Heparin plasma(n=5) 82-94% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS2/iNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 84-98% 95-105% 88-102%
EDTA plasma(n=5) 88-103% 83-94% 92-105% 89-97%
Heparin plasma(n=5) 87-102% 85-94% 87-101% 89-99%
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