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ELK Biotechnology

ELK1343

Human IL1z(Interleukin 1 Zeta) ELISA Kit

Human IL1z(Interleukin 1 Zeta) ELISA Kit

$376.00 USD
$376.00 USD
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Size
Sensitivity
Detection range

Human IL1z(Interleukin 1 Zeta) ELISA Kit

Overview

Product name: Human IL-37(Interleukin 37) ELISA Kit
Reactivity: Human
Alternative Names: IL-1Z;IL1 Zeta; IL1F7; IL37; FIL1; FIL1(ZETA); FIL1Z; IL-1F7; IL-1H4; IL-1RP1; IL1H4; IL1RP1; Interleukin 37; Interleukin-1-Related Protein; Interleukin 1 Family; Member 7
Assay Type: Sandwich
Sensitivity: 3.3 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
500.00 2.172 2.074
250.00 1.588 1.490
125.00 1.106 1.008
62.50 0.835 0.737
31.25 0.539 0.441
15.63 0.384 0.286
7.82 0.247 0.149
0.00 0.098 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-37 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-37 in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 81-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-37 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 93-105% 91-99% 83-96%
EDTA plasma(n=5) 79-91% 87-96% 95-103% 85-91%
Heparin plasma(n=5) 85-92% 90-96% 95-102% 87-98%
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