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ELK Biotechnology
SKU:ES8834
Thioredoxin Rabbit Polyclonal Antibody
Thioredoxin Rabbit Polyclonal Antibody
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$248.00 USD
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Thioredoxin Rabbit Polyclonal Antibody
Overview
| Product name: | Thioredoxin rabbit pAb |
| Alternative Names: | Thioredoxin (Trx;ATL-derived factor;ADF;Surface-associated sulphydryl protein;SASP) |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000 |
| Immunogen: | Synthesized peptide derived from human Thioredoxin. at AA range: 52-101 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 12kD |
| GeneID(Human): | 7295 |
| Human Swiss-Prot No: | P10599 |
| Cellular localization: | Nucleus . Cytoplasm . Secreted . Translocates from the cytoplasm into the nucleus after phorbol 12-myristate 13-acetate induction (PMA) (PubMed:9108029). Predominantly in the cytoplasm in non irradiated cells (PubMed:11118054). Radiation induces translocation of TRX from the cytoplasm to the nucleus (PubMed:11118054). Secreted by a leaderless secretory pathway (PubMed:1332947). . |
| Background: | The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of 1,mouse-liver 2,hela cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour) -
Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:400(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). -
Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:400(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). -
Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:400(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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