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ELK Biotechnology
SKU:ES8630
Keratin-pan Rabbit Polyclonal Antibody
Keratin-pan Rabbit Polyclonal Antibody
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Keratin-pan Rabbit Polyclonal Antibody
Overview
| Product name: | Keratin-pan rabbit pAb |
| Applications: | WB;ELISA;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen: | Synthetic peptide from human protein at AA range: 150-220 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 70kD |
| GeneID(Human): | 3849 |
| Human Swiss-Prot No: | P35908/Q01546/P12035/P13647/P02538/P04259/P48668/Q3SY84/Q14CN4/Q86Y46/Q7RTS7/O95678/Q5XKE5/P08729/P05787/Q9NSB2 |
| Cellular localization: | extracellular space,nucleus,cytoplasm,Golgi apparatus,intermediate filament,membrane,keratin filament,intermediate filament cytoskeleton,extracellular exosome, |
| Background: | keratin 2(KRT2) Homo sapiens The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western blot analysis of mouse-lung mouse-brain mouse-kidney mouse-heart lysate, antibody was diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 -
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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