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ELK Biotechnology
SKU:ES8529
E2F-1 Rabbit Polyclonal Antibody
E2F-1 Rabbit Polyclonal Antibody
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E2F-1 Rabbit Polyclonal Antibody
Overview
| Product name: | E2F-1 rabbit pAb |
| Alternative Names: | E2F1 RBBP3 |
| Applications: | IF;WB;IHC;ELISA |
| Recommended Dilutions: | IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300 |
| Immunogen: | Synthetic peptide from human protein at AA range: 100-170 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 60kD |
| GeneID(Human): | 1869 |
| Human Swiss-Prot No: | Q01094 |
| Cellular localization: | Nucleus . |
| Background: | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media |
| Species Reactivity: | Human;Mouse;Rat |
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Immunofluorescence analysis of Hela cell. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). HER2 Monoclonal Antibody(11H9)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:10 -
Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture -
Immunofluorescence analysis of rat-heart tissue. 1,E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture -
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,E2F-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tem
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