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ELK Biotechnology
SKU:ES8354
MRP-L35 Rabbit Polyclonal Antibody
MRP-L35 Rabbit Polyclonal Antibody
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MRP-L35 Rabbit Polyclonal Antibody
Overview
| Product name: | MRP-L35 rabbit pAb |
| Alternative Names: | MRPL35; BM-007; 39S ribosomal protein L35, mitochondrial; L35mt; MRP-L35 |
| Applications: | WB;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen: | Synthesized peptide derived from MRP-L35 . at AA range: 80-160 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 21kD |
| GeneID(Human): | 51318 |
| Human Swiss-Prot No: | Q9NZE8 |
| Cellular localization: | Mitochondrion . |
| Background: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse |
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Western blot analysis of 293T COLO using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 -
Western blot analysis of 293 using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 -
Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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