TBC1D4; AS160; KIAA0603; TBC1 domain family member 4; Akt substrate of 160 kDa; AS160
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human AS160 around the phosphorylation site of Thr642. AA range:611-660
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
150kD
GeneID（Human）:
9882
Human Swiss-Prot No:
O60343
Cellular localization:
Cytoplasm . Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
Background:
This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Ph
Western blot analysis of L929 using p-TBC1D4 (T642) antibody. Antibody was diluted at 1:2000
Immunofluorescence analysis of HeLa cells, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide.