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ELK Biotechnology
SKU:ES8084
eIF5B Rabbit Polyclonal Antibody
eIF5B Rabbit Polyclonal Antibody
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$248.00 USD
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eIF5B Rabbit Polyclonal Antibody
Overview
| Product name: | eIF5B rabbit pAb |
| Alternative Names: | EIF5B; IF2; KIAA0741; Eukaryotic translation initiation factor 5B; eIF-5B; Translation initiation factor IF-2 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human EIF5B. AA range:1051-1100 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 139kD |
| GeneID(Human): | 9669 |
| Human Swiss-Prot No: | O60841 |
| Cellular localization: | Cytoplasm . |
| Background: | Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor -
Western blot analysis of lysates from Jurkat, HepG2, and A549 cells, using EIF5B Antibody. The lane on the right is blocked with the synthesized peptide.
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