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ELK Biotechnology
SKU:ES8082
CEP135 Rabbit Polyclonal Antibody
CEP135 Rabbit Polyclonal Antibody
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CEP135 Rabbit Polyclonal Antibody
Overview
| Product name: | CEP135 rabbit pAb |
| Alternative Names: | CEP135; CEP4; KIAA0635; Centrosomal protein of 135 kDa; Cep135; Centrosomal protein 4 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CEP135. AA range:1081-1130 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 134kD |
| GeneID(Human): | 9662 |
| Human Swiss-Prot No: | Q66GS9 |
| Cellular localization: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles. |
| Background: | centrosomal protein 135(CEP135) Homo sapiens This gene encodes a centrosomal protein, which acts as a scaffolding protein during early centriole biogenesis, and is also required for centriole-centriole cohesion during interphase. Mutations in this gene are associated with autosomal recessive primary microcephaly-8. [provided by RefSeq, Jun 2012], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of COLO cells using CEP135 Polyclonal Antibody diluted at 1:1000 -
Western blot analysis of mouse-lung mouse-heart 293T lysis using CEP135 antibody. Antibody was diluted at 1:1000 -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Western blot analysis of lysates from COLO cells, using CEP135 Antibody. The lane on the right is blocked with the synthesized peptide.
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