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ELK Biotechnology
SKU:ES8032
CD28 (phospho Tyr218) Rabbit Polyclonal Antibody
CD28 (phospho Tyr218) Rabbit Polyclonal Antibody
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CD28 (phospho Tyr218) Rabbit Polyclonal Antibody
Overview
| Product name: | CD28 (phospho Tyr218) rabbit pAb |
| Alternative Names: | CD28; T-cell-specific surface glycoprotein CD28; TP44; CD antigen CD28 |
| Applications: | WB;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218. AA range:171-220 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 45kD |
| GeneID(Human): | 940 |
| Human Swiss-Prot No: | P10747 |
| Cellular localization: | Membrane; Single-pass type I membrane protein.; [Isoform 3]: Cell surface . |
| Background: | The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011], |
| Species Reactivity: | Human |
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD28 (Phospho-Tyr218) Antibody -
Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using CD28 (Phospho-Tyr218) Antibody. The lane on the right is blocked with the phospho peptide.
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