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ELK Biotechnology
SKU:ES7983
CD3 ζ (phospho Tyr142) Rabbit Polyclonal Antibody
CD3 ζ (phospho Tyr142) Rabbit Polyclonal Antibody
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CD3 ζ (phospho Tyr142) Rabbit Polyclonal Antibody
Overview
| Product name: | CD3 ζ (phospho Tyr142) rabbit pAb |
| Alternative Names: | CD247; CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD antigen CD247 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CD3 zeta around the phosphorylation site of Tyr142. AA range:111-160 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 20kD |
| GeneID(Human): | 919 |
| Human Swiss-Prot No: | P20963 |
| Cellular localization: | Cell membrane ; Single-pass type I membrane protein. |
| Background: | The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat;Monkey |
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD3 zeta (Phospho-Tyr142) Antibody -
Western blot analysis of lysates from Jurkat cells treated with UV 15', using CD3 zeta (Phospho-Tyr142) Antibody. The lane on the right is blocked with the phospho peptide.
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