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ELK Biotechnology
SKU:ES7923
STAM2 (phospho Tyr192) Rabbit Polyclonal Antibody
STAM2 (phospho Tyr192) Rabbit Polyclonal Antibody
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STAM2 (phospho Tyr192) Rabbit Polyclonal Antibody
Overview
| Product name: | STAM2 (phospho Tyr192) rabbit pAb |
| Alternative Names: | STAM2; HBP; Signal transducing adapter molecule 2; STAM-2; Hrs-binding protein |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human STAM2 around the phosphorylation site of Tyr192. AA range:161-210 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 58kD |
| GeneID(Human): | 8986 |
| Human Swiss-Prot No: | O75886 |
| Cellular localization: | Cytoplasm . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . |
| Background: | The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of 3T3 cells using Phospho-STAM2 (Y192) Polyclonal Antibody -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using STAM2 (Phospho-Tyr192) Antibody -
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using STAM2 (Phospho-Tyr192) Antibody. The lane on the right is blocked with the phospho peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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