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ELK Biotechnology
SKU:ES7801
IKKγ (phospho Ser85) Rabbit Polyclonal Antibody
IKKγ (phospho Ser85) Rabbit Polyclonal Antibody
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IKKγ (phospho Ser85) Rabbit Polyclonal Antibody
Overview
| Product name: | IKKγ (phospho Ser85) rabbit pAb |
| Alternative Names: | IKBKG; FIP3; NEMO; NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1; IKKAP1; Inhibitor of nuclear factor kappa-B kinase subunit gamma; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; NF |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human IKK-gamma around the phosphorylation site of Ser85. AA range:51-100 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 48kD |
| GeneID(Human): | 8517 |
| Human Swiss-Prot No: | Q9Y6K9 |
| Cellular localization: | Cytoplasm . Nucleus . Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress. . |
| Background: | This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western blot analysis of lysates from HepG2 cells treated with Anisomycin 0.5uM 5h, using IKK-gamma (Phospho-Ser85) Antibody. The lane on the right is blocked with the phospho peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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