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ELK Biotechnology
SKU:ES7777
COX4I2 Rabbit Polyclonal Antibody
COX4I2 Rabbit Polyclonal Antibody
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COX4I2 Rabbit Polyclonal Antibody
| Product name: | COX4I2 rabbit pAb |
| Alternative Names: | COX4I2; COX4L2; Cytochrome c oxidase subunit 4 isoform 2; mitochondrial; Cytochrome c oxidase subunit IV isoform 2; COX IV-2 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human COX42. AA range:31-80 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 20kD |
| GeneID(Human): | 84701 |
| Human Swiss-Prot No: | Q96KJ9 |
| Cellular localization: | Mitochondrion inner membrane ; Single-pass membrane protein . |
| Background: | Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of K562 insulin 0.01U/ml 15' cells using COX4I2 Polyclonal Antibody -
Immunofluorescence analysis of COS7 cells, using COX42 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from K562 cells, treated with insulin 0.01U/ml 15', using COX42 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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