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ELK Biotechnology
SKU:ES7755
Rad54 Rabbit Polyclonal Antibody
Rad54 Rabbit Polyclonal Antibody
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$248.00 USD
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Rad54 Rabbit Polyclonal Antibody
Overview
| Product name: | Rad54 rabbit pAb |
| Alternative Names: | RAD54L; RAD54A; DNA repair and recombination protein RAD54-like; RAD54 homolog; hHR54; hRAD54 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RAD54L. AA range:221-270 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 85kD |
| GeneID(Human): | 8438 |
| Human Swiss-Prot No: | Q92698 |
| Cellular localization: | Nucleus . |
| Background: | The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008], |
| Species Reactivity: | Human;Mouse |
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Western blot analysis of lysates from HeLa cells, Jurkat cells, and 293 cells, using RAD54 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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