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ELK Biotechnology
SKU:ES7753
NIFK (phospho Thr234) Rabbit Polyclonal Antibody
NIFK (phospho Thr234) Rabbit Polyclonal Antibody
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NIFK (phospho Thr234) Rabbit Polyclonal Antibody
Overview
| Product name: | NIFK (phospho Thr234) rabbit pAb |
| Alternative Names: | MKI67IP; NIFK; NOPP34; MKI67 FHA domain-interacting nucleolar phosphoprotein; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK |
| Applications: | IHC;IF;ELISA |
| Recommended Dilutions: | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NIFK around the phosphorylation site of Thr234. AA range:200-249 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| GeneID(Human): | 84365 |
| Human Swiss-Prot No: | Q9BYG3 |
| Cellular localization: | Nucleus, nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67. |
| Background: | This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009], |
| Species Reactivity: | Human;Mouse |
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NIFK (Phospho-Thr234) Antibody -
Immunofluorescence analysis of HUVEC cells, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide. -
Immunohistochemistry analysis of paraffin-embedded human brain, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.
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