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ELK Biotechnology
SKU:ES7749
PRAS40 (phospho Thr246) Rabbit Polyclonal Antibody
PRAS40 (phospho Thr246) Rabbit Polyclonal Antibody
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PRAS40 (phospho Thr246) Rabbit Polyclonal Antibody
Overview
| Product name: | PRAS40 (phospho Thr246) rabbit pAb |
| Alternative Names: | AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Akt1 S1 around the phosphorylation site of Thr246. AA range:207-256 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 40kD |
| GeneID(Human): | 84335 |
| Human Swiss-Prot No: | Q96B36 |
| Cellular localization: | Cytoplasm, cytosol . Found in the cytosolic fraction of the brain. . |
| Background: | AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Akt1 S1 (Phospho-Thr246) Antibody -
Western blot analysis of lysates from HepG2 cells treated with PDGF 50ng/ml 30', using Akt1 S1 (Phospho-Thr246) Antibody. The lane on the right is blocked with the phospho peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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