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ELK Biotechnology
SKU:ES7673
Histone H2A (phospho Thr121) Rabbit Polyclonal Antibody
Histone H2A (phospho Thr121) Rabbit Polyclonal Antibody
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Histone H2A (phospho Thr121) Rabbit Polyclonal Antibody
Overview
| Product name: | Histone H2A (phospho Thr121) rabbit pAb |
| Alternative Names: | HIST1H2AG; H2AFP; HIST1H2AI; H2AFC; HIST1H2AK; H2AFD; HIST1H2AL; H2AFI; HIST1H2AM; H2AFN; Histone H2A type 1; H2A.1; Histone H2A/p; HIST1H2AB; H2AFM; HIST1H2AE; H2AFA; Histone H2A type 1-B/E; Histone H2A.2; Histone H2A/a; Histone H2A/m; HIS |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | WB 1:500-2000 ,Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Histone H2A around the phosphorylation site of Thr121. AA range:81-130 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| GeneID(Human): | 8329/8332/8336/8969/3012/8335/3013/85235/723790/8337/92815 |
| Human Swiss-Prot No: | P0C0S8/P04908/P20671/Q96KK5/Q6FI13/Q7L7L0 |
| Cellular localization: | Nucleus. Chromosome. |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of Hela+UV 5' cells using Phospho-Histone H2A (T121) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Histone H2A (Phospho-Thr121) Antibody -
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Histone H2A (Phospho-Thr121) Antibody. The picture on the right is blocked with the phospho peptide.
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