Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human FZD6. AA range:116-165
Storage:
-20°C/1 year
Storage:
Rabbit
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
79kD
GeneID（Human）:
8323
Human Swiss-Prot No:
O60353
Cellular localization:
Membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Cell surface . Apical cell membrane; Multi-pass membrane protein . Cytoplasmic vesicle membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Colocalizes with FZD3 at the apical face of cells (By similarity). Localizes to the endoplasmic reticulum membrane in the presence of LMBR1L (By similarity). .
Background:
frizzled class receptor 6(FZD6) Homo sapiens This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.[provided by RefSeq, Aug 2011],
Immunofluorescence analysis of MCF7 cells, using FZD6 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using FZD6 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of FZD6 Antibody. The lane on the right is blocked with the FZD6 peptide.