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ELK Biotechnology
SKU:ES7657
CAF-1 p60 Rabbit Polyclonal Antibody
CAF-1 p60 Rabbit Polyclonal Antibody
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CAF-1 p60 Rabbit Polyclonal Antibody
Overview
| Product name: | CAF-1 p60 rabbit pAb |
| Alternative Names: | CHAF1B; CAF1A; CAF1P60; MPHOSPH7; MPP7; Chromatin assembly factor 1 subunit B; CAF-1 subunit B; Chromatin assembly factor I p60 subunit; CAF-I 60 kDa subunit; CAF-I p60; M-phase phosphoprotein 7 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CAF1B. AA range:71-120 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 61kD |
| GeneID(Human): | 8208 |
| Human Swiss-Prot No: | Q13112 |
| Cellular localization: | Nucleus . Cytoplasm . DNA replication foci. Cytoplasmic in M phase. |
| Background: | Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Western blot analysis of lysates from Jurkat cells, using CAF1B Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from RAW264.7cells using CAF1B antibody.
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