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ELK Biotechnology
SKU:ES7527
14-3-3 β/ζ Rabbit Polyclonal Antibody
14-3-3 β/ζ Rabbit Polyclonal Antibody
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14-3-3 β/ζ Rabbit Polyclonal Antibody
Overview
| Product name: | 14-3-3 β/ζ rabbit pAb |
| Alternative Names: | YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAB; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human YWHAZ. AA range:151-200 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 24kD |
| GeneID(Human): | 7534/7529 |
| Human Swiss-Prot No: | P63104/P31946 |
| Cellular localization: | Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes. |
| Background: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western blot analysis of lysates from NIH/3T3 cells, using 14-3-3 beta/zeta Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:2
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