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ELK Biotechnology
SKU:ES7523
14-3-3 η Rabbit Polyclonal Antibody
14-3-3 η Rabbit Polyclonal Antibody
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14-3-3 η Rabbit Polyclonal Antibody
Overview
| Product name: | 14-3-3 η rabbit pAb |
| Alternative Names: | YWHAH; YWHA1; 14-3-3 protein eta; Protein AS1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human 14-3-3 eta. AA range:51-100 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 42kD |
| GeneID(Human): | 7533 |
| Human Swiss-Prot No: | Q04917 |
| Cellular localization: | cytoplasm,mitochondrion,cytosol,plasma membrane,intercalated disc,cytoplasmic vesicle membrane,extracellular exosome, |
| Background: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Immunofluorescence analysis of HeLa cells, using 14-3-3 eta Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from Jurkat cells, using 14-3-3 eta Antibody. The lane on the right is blocked with the synthesized peptide.
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