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ELK Biotechnology
SKU:ES7506
Vimentin (phospho Ser83) Rabbit Polyclonal Antibody
Vimentin (phospho Ser83) Rabbit Polyclonal Antibody
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Vimentin (phospho Ser83) Rabbit Polyclonal Antibody
Overview
| Product name: | Vimentin (phospho Ser83) rabbit pAb |
| Alternative Names: | VIM; Vimentin |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Vimentin around the phosphorylation site of Ser83. AA range:56-105 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 54kD |
| GeneID(Human): | 7431 |
| Human Swiss-Prot No: | P08670 |
| Cellular localization: | Cytoplasm . Cytoplasm, cytoskeleton . Nucleus matrix . Cell membrane . |
| Background: | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of 293 cells using Phospho-Vimentin (S83) Polyclonal Antibody -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vimentin (Phospho-Ser83) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from 293 cells treated with paclitaxel 1uM 24h, using Vimentin (Phospho-Ser83) Antibody. The lane on the right is blocked with the phospho peptide.
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