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ELK Biotechnology
SKU:ES7444
TPH1 Rabbit Polyclonal Antibody
TPH1 Rabbit Polyclonal Antibody
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$248.00 USD
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TPH1 Rabbit Polyclonal Antibody
Overview
| Product name: | TPH1 rabbit pAb |
| Alternative Names: | TPH1; TPH; TPRH; TRPH; Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Tryptophan Hydroxylase. AA range:231-280 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 51kD |
| GeneID(Human): | 7166 |
| Human Swiss-Prot No: | P17752 |
| Cellular localization: | cytosol,neuron projection, |
| Background: | This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009], |
| Species Reactivity: | Human;Mouse;Rat |
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Immunofluorescence analysis of Hela cell. 1,TPH1 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min. -
Western Blot analysis of HepG2 cells using TPH1 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 -
Immunofluorescence analysis of HUVEC cells, using Tryptophan Hydroxylase Antibody. The picture on the right is blocked with the synthesized peptide. -
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Tryptophan Hydroxylase Antibody. The picture on the right is blocked with the synthesized peptide.
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