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ELK Biotechnology
SKU:ES7397
Thymidine Kinase Rabbit Polyclonal Antibody
Thymidine Kinase Rabbit Polyclonal Antibody
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$248.00 USD
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Thymidine Kinase Rabbit Polyclonal Antibody
Overview
| Product name: | Thymidine Kinase rabbit pAb |
| Alternative Names: | TK1; Thymidine kinase; cytosolic |
| Applications: | WB;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human KITH. AA range:161-210 |
| Storage: | -20°C/1 year |
| Storage: | Rabbit |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 25kD |
| GeneID(Human): | 7083 |
| Human Swiss-Prot No: | P04183 |
| Cellular localization: | Cytoplasm. |
| Background: | catalytic activity:ATP + thymidine = ADP + thymidine 5'-phosphate.,miscellaneous:Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.,PTM:Phosphorylated on Ser-13 in mitosis.,similarity:Belongs to the thymidine kinase family., |
| Species Reactivity: | Human;Monkey |
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Western blot analysis of lysates from HeLa and COS7 cells, using KITH Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from RAW264.7cells using KITH antibody. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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