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ELK Biotechnology
SKU:ES7273
SRF (phospho Ser103) Rabbit Polyclonal Antibody
SRF (phospho Ser103) Rabbit Polyclonal Antibody
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SRF (phospho Ser103) Rabbit Polyclonal Antibody
Overview
| Product name: | SRF (phospho Ser103) rabbit pAb |
| Alternative Names: | SRF; Serum response factor; SRF |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human SRF around the phosphorylation site of Ser99. AA range:71-120 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 51kD |
| GeneID(Human): | 6722 |
| Human Swiss-Prot No: | P11831 |
| Cellular localization: | Nucleus . |
| Background: | This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014], |
| Species Reactivity: | Human;Mouse;Rat |
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using SRF (Phospho-Ser99) Antibody -
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using SRF (Phospho-Ser99) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from LOVO cells treated with Serum 10% 15', using SRF (Phospho-Ser99) Antibody. The lane on the right is blocked with the phospho peptide.
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