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ELK Biotechnology
SKU:ES7254
Sox-9 (phospho Ser181) Rabbit Polyclonal Antibody
Sox-9 (phospho Ser181) Rabbit Polyclonal Antibody
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Sox-9 (phospho Ser181) Rabbit Polyclonal Antibody
Overview
| Product name: | Sox-9 (phospho Ser181) rabbit pAb |
| Alternative Names: | SOX9; Transcription factor SOX-9 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human SOX-9 around the phosphorylation site of Ser181. AA range:147-196 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 65kD |
| GeneID(Human): | 6662 |
| Human Swiss-Prot No: | P48436 |
| Cellular localization: | Nucleus . |
| Background: | SRY-box 9(SOX9) Homo sapiens The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse |
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Western blot analysis of Sox-9 (phospho Ser181) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with UV, 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour. -
Immunofluorescence analysis of A549 cells, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide. -
Immunohistochemistry analysis of paraffin-embedded human brain, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from 293 cells treated with PBS 60', using SOX-9 (Phospho-Ser181) Antibody. The lane on the right is blocked with the phospho peptide.
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