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ELK Biotechnology
SKU:ES7222
p47-phox (phospho Ser359) Rabbit Polyclonal Antibody
p47-phox (phospho Ser359) Rabbit Polyclonal Antibody
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p47-phox (phospho Ser359) Rabbit Polyclonal Antibody
Overview
| Product name: | p47-phox (phospho Ser359) rabbit pAb |
| Alternative Names: | NCF1; NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human p47 phox around the phosphorylation site of Ser359. AA range:331-380 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 45kD |
| GeneID(Human): | 653361 |
| Human Swiss-Prot No: | P14598 |
| Cellular localization: | Cytoplasm, cytosol . Membrane ; Peripheral membrane protein ; Cytoplasmic side . |
| Background: | The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Rat;Mouse; |
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Western Blot analysis of HeLa nocodazole 1ug/ml 18h cells using Phospho-p47-phox (S359) Polyclonal Antibody diluted at 1:500 -
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbe -
Immunofluorescence analysis of HeLa cells, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is blocked with the phospho peptide. -
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is blocked with the phospho peptide.
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