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ELK Biotechnology
SKU:ES7152
ACOT1 Rabbit Polyclonal Antibody
ACOT1 Rabbit Polyclonal Antibody
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$248.00 USD
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ACOT1 Rabbit Polyclonal Antibody
Overview
| Product name: | ACOT1 rabbit pAb |
| Alternative Names: | ACOT1; CTE1; Acyl-coenzyme A thioesterase 1; Acyl-CoA thioesterase 1; CTE-I; CTE-Ib; Inducible cytosolic acyl-coenzyme A thioester hydrolase; Long chain acyl-CoA thioester hydrolase; Long chain acyl-CoA hydrolase |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ACOT1. AA range:91-140 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 46kD |
| GeneID(Human): | 641371 |
| Human Swiss-Prot No: | Q86TX2 |
| Cellular localization: | Cytoplasm, cytosol . |
| Background: | catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16.,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Monomer., |
| Species Reactivity: | Human;Rat;Mouse; |
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbe -
Immunofluorescence analysis of MCF7 cells, using ACOT1 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from Jurkat cells, using ACOT1 Antibody. The lane on the right is blocked with the synthesized peptide. -
Western blot analysis of the lysates from HT-29 cells using ACOT1 antibody.
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