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ELK Biotechnology
SKU:ES7132
NBK (phospho Thr33) Rabbit Polyclonal Antibody
NBK (phospho Thr33) Rabbit Polyclonal Antibody
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NBK (phospho Thr33) Rabbit Polyclonal Antibody
Overview
| Product name: | NBK (phospho Thr33) rabbit pAb |
| Alternative Names: | BIK; NBK; Bcl-2-interacting killer; Apoptosis inducer NBK; BIP1; BP4 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human BIK around the phosphorylation site of Thr33. AA range:18-67 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 30kD |
| GeneID(Human): | 638 |
| Human Swiss-Prot No: | Q13323 |
| Cellular localization: | Endomembrane system; Single-pass membrane protein. Mitochondrion membrane ; Single-pass membrane protein . Around the nuclear envelope, and in cytoplasmic membranes. |
| Background: | The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins. [provided by RefSeq, Sep 2011], |
| Species Reactivity: | Human;Rat;Mouse; |
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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorb -
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BIK (Phospho-Thr33) Antibody -
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using BIK (Phospho-Thr33) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from K562 cells, using BIK (Phospho-Thr33) Antibody. The lane on the right is blocked with the phospho peptide.
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