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ELK Biotechnology
SKU:ES7064
Ribosomal Protein L5 Rabbit Polyclonal Antibody
Ribosomal Protein L5 Rabbit Polyclonal Antibody
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Ribosomal Protein L5 Rabbit Polyclonal Antibody
Overview
| Product name: | Ribosomal Protein L5 rabbit pAb |
| Alternative Names: | RPL5; MSTP030; 60S ribosomal protein L5 |
| Applications: | WB;IP;IHC;IF;ELISA |
| Recommended Dilutions: | WB 1:500-2000;IP 1:50-200;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RPL5. AA range:161-210 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 34kD |
| GeneID(Human): | 6125 |
| Human Swiss-Prot No: | P46777 |
| Cellular localization: | Cytoplasm . Nucleus, nucleolus . |
| Background: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found |
| Species Reactivity: | Human;Mouse;Rat |
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Western blot analysis of lysates from Jurkat and HepG2 cells, using RPL5 Antibody. The lane on the right is blocked with the synthesized peptide.
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