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ELK Biotechnology
SKU:ES7043
RGS12 Rabbit Polyclonal Antibody
RGS12 Rabbit Polyclonal Antibody
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$248.00 USD
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RGS12 Rabbit Polyclonal Antibody
Overview
| Product name: | RGS12 rabbit pAb |
| Alternative Names: | RGS12; Regulator of G-protein signaling 12; RGS12 |
| Applications: | WB;ELISA;IHC |
| Recommended Dilutions: | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RGS12. AA range:141-190 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 160kD |
| GeneID(Human): | 6002 |
| Human Swiss-Prot No: | O14924 |
| Cellular localization: | Nucleus . Cytoplasm . Cell projection, dendrite . Cell junction, synapse .; [Isoform 5]: Nucleus matrix . Also localized to discrete nuclear foci that are distinct from sites of RNA processing, PML nuclear bodies, and PcG domains. . |
| Background: | This gene encodes a member of the 'regulator of G protein signaling' (RGS) gene family. The encoded protein may function as a guanosine triphosphatase (GTPase)-activating protein as well as a transcriptional repressor. This protein may play a role in tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their biological nature has not been determined. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of 3T3 cells using RGS12 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Western blot analysis of RGS12 Antibody. The lane on the right is blocked with the RGS12 peptide. -
Western blot analysis of the lysates from HUVECcells using RGS12 antibody. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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