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ELK Biotechnology
SKU:ES7018
Recoverin Rabbit Polyclonal Antibody
Recoverin Rabbit Polyclonal Antibody
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$248.00 USD
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Recoverin Rabbit Polyclonal Antibody
Overview
| Product name: | Recoverin rabbit pAb |
| Alternative Names: | RCVRN; RCV1; Recoverin; Cancer-associated retinopathy protein; Protein CAR |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Recoverin. AA range:107-156 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 23kD |
| GeneID(Human): | 5957 |
| Human Swiss-Prot No: | P35243 |
| Cellular localization: | Photoreceptor inner segment . Cell projection, cilium, photoreceptor outer segment . Photoreceptor outer segment membrane ; Lipid-anchor ; Cytoplasmic side . Perikaryon . Primarily expressed in the inner segments of light-adapted rod photoreceptors, approximately 10% of which translocates from photoreceptor outer segments upon light stimulation (By similarity). Targeting of myristoylated protein to rod photoreceptor outer segments is calcium dependent (By similarity). . |
| Background: | This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse |
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Western Blot analysis of 3T3 cells using Recoverin Polyclonal Antibody -
Western blot analysis of lysates from NIH/3T3 cells, using Recoverin Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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