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ELK Biotechnology
SKU:ES6995
Rb (phospho Thr826) Rabbit Polyclonal Antibody
Rb (phospho Thr826) Rabbit Polyclonal Antibody
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Rb (phospho Thr826) Rabbit Polyclonal Antibody
Overview
| Product name: | Rb (phospho Thr826) rabbit pAb |
| Alternative Names: | RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Thr826. AA range:601-650 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 110kD |
| GeneID(Human): | 5925 |
| Human Swiss-Prot No: | P06400 |
| Cellular localization: | Nucleus . During keratinocyte differentiation, acetylation by KAT2B/PCAF is required for nuclear localization. . |
| Background: | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008], |
| Species Reactivity: | Human;Mouse;Rat |
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Western blot analysis of RAT-musle using p-Rb (T826) antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). -
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i -
Immunofluorescence analysis of COS7 cells, using Retinoblastoma (Phospho-Thr826) Antibody. The picture on the right is blocked with the phospho peptide. -
Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using Retinoblastoma (Phospho-Thr826) Antibody. The lane on the right is blocked with the phospho peptide.
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