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ELK Biotechnology
SKU:ES6909
BAF250b Rabbit Polyclonal Antibody
BAF250b Rabbit Polyclonal Antibody
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$248.00 USD
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BAF250b Rabbit Polyclonal Antibody
Overview
| Product name: | BAF250b rabbit pAb |
| Alternative Names: | ARID1B; BAF250B; DAN15; KIAA1235; OSA2; AT-rich interactive domain-containing protein 1B; ARID domain-containing protein 1B; BRG1-associated factor 250b; BAF250B; BRG1-binding protein hELD/OSA1; Osa homolog 2; hOsa2; p250R |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human BAF250B. AA range:1371-1420 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 170kD |
| GeneID(Human): | 57492 |
| Human Swiss-Prot No: | Q8NFD5 |
| Cellular localization: | Nucleus . |
| Background: | This locus encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by this locus is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2012], |
| Species Reactivity: | Human;Rat |
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Western Blot analysis of 3T3 cells using BAF250b Polyclonal Antibody diluted at 1:1000 -
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor -
Immunofluorescence analysis of HUVEC cells, using BAF250B Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from LOVO cells, using BAF250B Antibody. The lane on the right is blocked with the synthesized peptide.
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